Dynamic changes in Hsf1 phosphorylation during the heat shock response.

<p>Comparison of model simulations and experimental evidence depicting Hsf1activation during heat shock. (A) Model simulations of the dynamic changes in Hsf1 phosphorylation levels following heat shock: grey line 30°C–37°C, black line: 30°C–42°C. (B) Graphical illustration of experimentally determined Hsf1 phosphorylation levels following heat shock from experiments such as those shown in (C) and (D): grey line 30°C–37°C, black line: 30°C–42°C. (C) The upper panel shows Hsf1 phosphorylation levels during a 30°C–37°C heat shock. The lower panel shows lambda phosphatase controls (λ Ppase) that confirm that Hsf1 is phosphorylated at the 2, 5 and 10 minute time points in the upper panel. (D) The upper panel shows Hsf1 phosphorylation levels during a 30°C–42°C heat shock. The lower panel shows lambda phosphatase controls (λ Ppase) that confirm Hsf1 phosphorylation for the 5, 30 and 120 minute time points in the upper panel. White and black arrows depict the unphosphorylated and phosphorylated forms respectively. Data reflect the outcomes for at least three independent replicate experiments.