Mass Spectrometry for Amino Acid Sequence Verification of the Deduced CRY Protein.

<p>Protein samples were digested with gluC and trypsin proteases separately and analyzed using high resolution LC MS/MS. Mass spectrometry verified 97% of the deduced recombinant CRY sequence. The remaining 3% was either unidentified or represented 1 amino acid mismatch (*). Blue shading = CyPet sequence; Red shading = RBP sequence; Yellow shading = YPet sequence; Gray = linker sequences; Black Lines = His₆ and Xpress™ tags.