Ectopic HCs in cochlear spiral ganglion and outer sulcus region.

<p>(<b>A–B′</b>) Triple staining of TUJ1, Myosin-VI, and EGFP. (B) The high-magnification image of the square area in (A). (B′) The image of confocal XZ plane through the dashed line in (B). Arrows target the same ectopic HC in (B) and (B′). (<b>C</b>) Double staining image of Synaptophysin (a synaptic marker) and Myosin-VI in the cochlear spiral ganglion region. Very adjacent to the new HC (inside the dotted white circle) lies the Synaptophysin+ dot, suggesting presence of the synaptic structure. (<b>D–E</b>) Images of samples stained with Myosin-VI antibody in experimental (D) and control (E) cochlear samples. Ectopic HCs were present in outer sulcus regions in the experimental but not the control group. OC: organ of Corti; OHCs: outer hair cells; IHCs: inner hair cells; XY: Confocal XY plane; XZ: Confocal XZ plane. Scale bars: 200 µm in (A) and 20 µm in (B, D) and 10 µm (C).