Treg depletion causes an increase in virus-specific CD8+ T cells in the brain.

<p>(<b>A</b>) The proportion of virus-specific D^bMV-H_22–30-pentamer⁺ CD8⁺ T cells of all CD8⁺ T cells was determined in spleen, lymph nodes, and brains of MV-infected C57BL/6 mice at days 3, 7, 10, 14, and 28 post infection (n = 3). MV-specific cells were gated as CD19-negative lymphocytes to exclude pentamer⁺ CD19⁺ cells. The total number of CD8⁺ T cells (<b>B</b>) and the number and proportion of D^bMV-H_22–30-pentamer⁺ CD8⁺ T cells (<b>C</b>) was determined in brains of 28 days infected control (DEREG^−/−) and DEREG (DEREG^−/+) mice, both treated with DT (Values ± SEM; n = 3).