Increased fMLP-induced changes of cytosolic calcium in monocytes from patients with essential hypertension.

<p><b>A</b>, <b>B</b>; Representative fluorescence tracings in monocytes from normotensive control subjects (<b>A</b>) and hypertensive patients (<b>B</b>) after administration of fMLP (100 nm/L) in the absence and presence of TRP channel-inhibitor 2-APB. <b>C</b>; Summary data of changes of intracellular calcium concentration in monocytes from normotensive control subjects (NT; open bars) and patients with essential hypertension (HT; filled bars) by several doses of fMLP (10 nmol/L, 50 nmol/L, and 100 nm/L). Each n = 10; *p<0.05 for the comparison HT vs. NT. <b>D</b>, <b>E</b>; fMLP-induced cation influx into monocytes indicated by quenching of fura-2 by manganese (Mn²⁺, 1 µmol/L). Representative raw data, showing mamganese quenching with a stimulus (fMLP, 100 nmol/L) or without stimulus (control, w/o fMLP) in monocytes from normotensive control subjects (<b>D</b>; open black or grey circle; NT) and patients with essential hypertension (<b>E</b>; filled black or grey circle; HT). <b>F</b>; Bar graph shows the summary data of manganese quenching rate obtained from normotensive control subjects (NT, n = 6) and patients with essential hypertension (HT, n = 8) under the control conditions or after administration of fMLP (100 nmol/L). *p<0.05 or **p<0.01 for the comparison with their controls; and #p<0.05 or ## p<0.01 for the comparison HT (filled bars) vs. NT (open bars).