Dose-dependent differential binding of FGF21, FGF19 and FGF1 to FGFR1-KLB and FGFR4-KLB.

<p>(A) Competition ability of FGF21 and FGF1 with FGF19 to bind FGFR-KLB. Graded concentrations as indicated for FGF21 (open square), FGF19 (filled triangle) and FGF1 (filled circle) were added together with 2 ng/ml ¹²⁵I-FGF19 to the cells expressing FGFR1-KLB (solid line) or FGFR4-KLB (dot line) after 30 ng/ml Tet induction overnight, the remaining specific bindings of ¹²⁵I-FGF19 were determined as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0033870#pone-0033870-g001" target="_blank">Figure 1A</a>. (B) Competition ability of FGF21 with FGF1 to bind FGFR1-KLB. Graded concentrations as indicated for FGF21 (open square), FGF19 (not shown) and FGF1 (filled circle) were added together with 2 ng/ml ¹²⁵I-FGF1 to the cells expressing FGFR1-KLB (solid line) or FGFR4-KLB (Inset), the remaining specific bindings of ¹²⁵I-FGF1 under these conditions were determined as described above.