<i>trio</i> mutations affect dendritic branching of Class I vpda neurons cell autonomously.

<p><b>a–c</b>) A vpda neuron from a wandering third instar larvae of (<b>a</b>) <i>trio</i>¹ Gal4^2–21UAS-GFP/+ (control), (<b>b</b>) <i>trio</i>¹Gal4^2–21UAS-GFP/<i>trio</i>^M89 and (<b>c</b>) <i>trio</i>¹Gal4^2–21UAS-GFP/UAS-<i>trio</i> RNAi; scale bar 50 µm. <b>d–e</b>) Quantification of dendritic branching in control, <i>trio</i> mutant and UAS-<i>trio</i> RNAi expressing vpda neurons. <b>d</b>) Dendritic branching of vpda neuron: <i>trio</i> knockdown reduces number of dendritic branches, <b>e</b>) average number of branches per branch order of vpda neuron: <i>trio</i> knockdown affects number of only higher order branches. <b>f–g</b>) Quantification of dendritic length in control and <i>trio</i> mutant vpda neuron. <b>f</b>) Total dendritic length of vpda neuron: <i>trio</i> mutations reduce total dendritic length <b>g</b>) average dendritic length per branch order of vpda neuron: <i>trio</i> mutations do not affect average dendritic length. The number of samples (n value) for each genotype is indicated by the number inside the respective bar, error bars represent standard error of the mean (SEM) and asterisks indicate p value<0.05.