Preparation and characterization of tumor-derived NVs.

<p><b>A.</b> Western blot analysis of the melanoma antigen tyrosinase in fractions of tumor-derived NVs obtained from the Optiprep density gradients. The density of each fraction was determined by measuring absorbance at 340 nm. <b>B.</b> Transmission electron micrograph of the NVs prepared from autologous melanoma tissues. Bar, 200 nm. <b>C.</b> Size distribution of NVs, measured by dynamic light-scattering, shows a diameter range of 80–150 nm. <b>D.</b> Western blot detection of tyrosinase, melan-A, and histone in 10 µg of whole cell lysate (WC), exosomes (EX), and NV.