Expression analysis of shuffled promoters in tobacco protoplast transient assay.

<p>Fifty shuffled-promoters randomly selected from each of the six libraries (LssF, LssFS, LmsFSF, LmsFFS, LhsFuasFScp and LhsFSuasFcp) were fused with GUS reporter gene. These shuffled promoter-GUS constructs were evaluated along with promoter-GUS constructs of CaMV35S and parent (F and FS) promoters in tobacco protoplast transient assay as described in “<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0031931#s2" target="_blank">Materials and Methods</a>”. The average GUS activity (n mole MU/min/mg protein) of three replicates was presented in the histogram.