<p>Exemplar flow cytometry analysis. A) MVs derived from a direct co-culture of MSCs and NP cells (one cell population was DiL labeled) and incubated with the unlabeled cell population. B) MV-free supernatant after ultracentrifugation. C) Conditioned medium derived from a direct co-culture of MSCs and NP cells (one cell population was DiL labeled). Abbreviations: MSC: mesenchymal stem cell; NP: nucleus pulposus; MV: microvesicles; SN: supernatant; CM: conditioned medium; CFDA: 5,6 caboxyfluorescein diactetae, succinimidyl ester; DiL: Vybrant CM-DiL cell-labeling solution.</p
Running Head: Large-scale expansion of mouse ES cells 2 A large-scale stirred culture system for the...
<p>(A). E was fractionated over a Superdex® peptide column. <i>Mixl1:GFP</i> ES cells were cultured ...
<p>Confocal microscopy of SD-MSCs cultured in (A–C) Integra and (D–F) Pelnac. (A and E) DAPI nuclear...
<p>A–C: Exemplar flow cytometry to quantify transfer of membrane (DiL) components after 7 days. A) D...
<p>A–C: An exemplar flow cytometry analysis for CFDA labeled MSCs and SNARF labeled NP cells after 7...
International audienceTransplantation of mesenchymal stem/stromalcells (MSCs) has emerged as an effe...
PurposeDuring in vivo stem cell differentiation, mature cells often induce the differentiation of ne...
<p>Newly isolated human Mesenchymal stem cells (MSCs) were cultured in stem cell growth media for up...
<p>Cells were cultured for 3–4 passages, harvested, and analyzed by a flow cytometry system. The y-a...
<p>Vybrant CM-Dil labeled HSC-3 (red) and Vybrant DiO labeled Mfs (green) were co-cultured for up to...
<p>A – A schematic representation of the initial steps in establishing a SP expansion culture. ♀ C2C...
<p>After culture on matrigel in mTESR1 media undifferentiated hESCs were shifted to MP specific medi...
<p>(A, B) Fluorescent microscopy where MDC (unlabeled) and MSC (GFP+, labeling efficiency less than ...
We design a microfluidic patterned co-culture system for mouse mesenchymal stem cells mMSCs and neur...
<p>(A)-(C): fluorescent microscopy of a hMSC culture 24 hours after labeling with MC03F microparticl...
Running Head: Large-scale expansion of mouse ES cells 2 A large-scale stirred culture system for the...
<p>(A). E was fractionated over a Superdex® peptide column. <i>Mixl1:GFP</i> ES cells were cultured ...
<p>Confocal microscopy of SD-MSCs cultured in (A–C) Integra and (D–F) Pelnac. (A and E) DAPI nuclear...
<p>A–C: Exemplar flow cytometry to quantify transfer of membrane (DiL) components after 7 days. A) D...
<p>A–C: An exemplar flow cytometry analysis for CFDA labeled MSCs and SNARF labeled NP cells after 7...
International audienceTransplantation of mesenchymal stem/stromalcells (MSCs) has emerged as an effe...
PurposeDuring in vivo stem cell differentiation, mature cells often induce the differentiation of ne...
<p>Newly isolated human Mesenchymal stem cells (MSCs) were cultured in stem cell growth media for up...
<p>Cells were cultured for 3–4 passages, harvested, and analyzed by a flow cytometry system. The y-a...
<p>Vybrant CM-Dil labeled HSC-3 (red) and Vybrant DiO labeled Mfs (green) were co-cultured for up to...
<p>A – A schematic representation of the initial steps in establishing a SP expansion culture. ♀ C2C...
<p>After culture on matrigel in mTESR1 media undifferentiated hESCs were shifted to MP specific medi...
<p>(A, B) Fluorescent microscopy where MDC (unlabeled) and MSC (GFP+, labeling efficiency less than ...
We design a microfluidic patterned co-culture system for mouse mesenchymal stem cells mMSCs and neur...
<p>(A)-(C): fluorescent microscopy of a hMSC culture 24 hours after labeling with MC03F microparticl...
Running Head: Large-scale expansion of mouse ES cells 2 A large-scale stirred culture system for the...
<p>(A). E was fractionated over a Superdex® peptide column. <i>Mixl1:GFP</i> ES cells were cultured ...
<p>Confocal microscopy of SD-MSCs cultured in (A–C) Integra and (D–F) Pelnac. (A and E) DAPI nuclear...