Calpastatin overexpression increases neuronal but not glial differentiation.

<p>β-III Tubulin and GFAP expression levels were evaluated by immunoblotting in NS-TGFP cells. Cells were transfected with either pcDNA-Flag-mCAST or pcDNA empty vector (control), differentiated and collected after 24 h, as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0033468#s4" target="_blank"><i>Materials and Methods</i></a>. (<i>A</i>) Representative immunoblots of Flag expression and β-actin in control and calpastatin overexpressing cells. (<i>B</i>) Representative immunoblots (top) and corresponding densitometry analysis (bottom) showing increased β-III Tubulin expression (left) and unchanged GFAP (right) protein levels in pcDNA-Flag-mCAST transfected cells. β-actin was used as loading control. Results are expressed as mean ± SEM arbitrary units for six independent experiments. *<i>p</i><0.01 from control.