Comparative expression analysis (in Agro-infiltration assay) of selected shuffled promoters screened in protoplast transient assay.

<p>In histogram shown, twenty six shuffled promoters giving good activity in transient tobacco protoplast assay selected from six libraries: 4 from LssF, 3 from LssFS, 2 from LmsFFS, 6 from LmsFSF, 4 from LhsFSuasFScp and 7 from LhsFuasFScp; were taken for further comparative expression analysis along with the CaMV35S promoter (35S), parent promoters (F and FS) and hybrid promoters (FuasFScp and FSuasFcp) in Agro-infiltration experiment using whole tobacco plants (<i>Nicotiana tabacum</i> samsun NN) as described in “<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0031931#s2" target="_blank">Materials and Methods</a>”. The average GUS activity (n mole MU/min/mg protein ± SD) of three replicates of each construct was presented in the histogram. Error bar shows the 95% confidence intervals of the mean. Statistical (one-way analysis of variance, ANOVA) analysis showed an extremely significant <i>P</i> value of <0.001. Empty vector with no GUS gene was treated as ‘Control’.