Granzyme B-Elispot of NK92 cells (A) or isolated NK cells (B,C) after 24 hours of co-culture with i/uRBC.

<p>Some cultures were pre-activated 5 days with TKD peptide or the scrambled NGL peptide and/or pre-incubated with blocking Hsp70 antibody (cmHsp70.2) or a blocking antibody IgM-isotype control for 20 minutes before the start of the experiment. After stimulation, 2000 NK cells were cultured either alone, with iRBC or uRBC (1∶3 or 10∶1) on a GzmB antibody-coated 96-well plate. GzmB-releasing cells were counted after 24 hours of incubation (* p≤0.05, ** p≤0.01, *** p≤0.001, student's <i>t</i> test, n = 6).