Analysis of physiological mitochondrial superoxide production using MitoSOX Red.

<p>The representative fluorescence images showed the oxidized MitoSOX fluorescence signal in control H9c2 cells (A) and H9c2 cells following 24 treatment with 100 nM Qter® (B). The fluorescence intensity reported in panel C was quantified by Image J software. Values are presented as means ± SD; <i>n</i> = 20. * p≤0.001.