VP53A interacted with Y455 clone in yeast.

<p>(A) Yeast representing protein-protein interactions grew on high-stringency (–Leu/–Trp/–His/–Ade) medium. Plasmids used in this figure are described in the <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0033216#s4" target="_blank">Materials and Methods</a>. The blue signal in image was due to the presence of X-α-Gal. (B) Far western blot. Upper image: the yeast lysates were transferred on PVDF membrane and then detected with anti-HA antibody. Lower image: the yeast lysates were transferred on PVDF membrane, incubated with recombinant VP53A and the detected with anti-VP53A antibody. The yeast transformed with pGADT7-Rec only was set as control.