Comparative expression analysis of promoters and promoter fragments fused with reporter genes (GFP and GUS) using CLSM in tobacco transient protoplast assay.

<p>(a) GFP constructs of CaMV35S promoter, parent promoters (F and FS), hybrid promoters (FuasFScp and FSuasFcp), promoter fragments (FScp, Fcp, FSuas and Fuas) were created as described in “<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0031931#s2" target="_blank">Materials and Methods</a>”. The GFP fluorescence intensity (in gray scale unit) was measured in protoplast transient assay using CLSM as described in “<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0031931#s2" target="_blank">Materials and Methods</a>”. The average GFP intensity ± SD of two replicates of each construct was presented in the histogram. Error bar shows the 95% confidence intervals of the mean. Statistical (one-way analysis of variance, ANOVA) analysis showed an extremely significant <i>P</i> value of <0.01. Empty vector ‘Control’ with no GFP gene was shown. (b) GUS constructs of CaMV35S promoter, parent promoters (F and FS), hybrid promoters (FuasFScp and FSuasFcp), and promoter fragments (FScp, Fcp, FSuas and Fuas) were generated as described in“<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0031931#s2" target="_blank">Materials and Methods</a>”. The GUS activity (n mole MU/min/mg protein) was measured in protoplast transient assay using CLSM as described in “<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0031931#s2" target="_blank">Materials and Methods</a>”. The average GUS activity ± SD of two replicates of each construct was presented in the histogram. Error bar shows the 95% confidence intervals of the means. Statistical (one-way analysis of variance, ANOVA) analysis showed an extremely significant <i>P</i> value of <0.02. Empty vector ‘Control’ with no GUS gene was shown.