Comparative expression analysis of hybrid promoters (FuasFScp and FSuasFcp), and CaMV 35S promoter fused with human alpha defensin-1 (HNP-1) gene in tobacco protoplasts.

<p>The human alpha defensin-1 (HNP-1) gene was expressed in protoplasts under the control of CaMV 35S, FuasFScp and FSuasFcp promoters as described in “<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0031931#s2" target="_blank">Materials and Methods</a>”. Antibacterial assay of human alpha defensin-1 (HNP-1) protein extracted from tobacco protoplasts was performed using (a) <i>Escherichia coli</i> cell and (b) <i>Staphylococcus aureus</i> cell as described in “<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0031931#s2" target="_blank">Materials and Methods</a>” section. The data were presented as a mean of two independent experiments with respective SD.