Hematoxylin and Eosin stained dorso lateral prostate of TRAMP mice.

<p><b>A</b>, H&E stained section of DLP of Group 2 control (i) and UA-treated (ii) TRAMP prostate. Arrow indicates LG-PIN, notched right arrow indicates HG-PIN, and block right arrow indicates WDC. (iii) Bar chart shows the incidence of LG-PIN, HG-PIN, WDC, MDC and PDC in DLP of control and Group 2 mice. Reductions in PIN, WDC and MDC were observed after UA treatment. <b>B</b>, H&E stained section of DLP of Group 3 control (i) and UA-treated (ii) TRAMP prostate. As described in A; Arrow head indicates MDC and chevron indicates PDC. <b>C</b>, Western blot of pSTAT3, pAKT, pIKKα/β, and p65 proteins in DLP in Groups 1, 2 and 3 mice. DLP lysates was prepared and immunoblotting was performed as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0032476#s4" target="_blank">Materials and Methods</a>. Equal protein loading for pAKT and pIKKα/β was determined by stripping and probing for total AKT and IKKα, p65 and STAT3 blots were stripped and reprobed for β-actin to determine equal protein loading. The band density was quantitated using Image J software. NT = non-TRAMP-mice; T = TRAMP controls; T+UA = UA-treated TRAMP-mice.