DSS-associated molecules do not affect the resistance of Caco2-BBE monolayers.

<p>Confluent cultures of Caco2-BBE cells were obtained after 48 h (dark lines) and epithelial resistance was assessed by conduct of continuous resistance measurements at pH 7.4 (cellular pH) and pH 6.2 (colonic pH). Resistance (Ohm) of Caco2-BBE cells was measured at Ω = 500 Hz and V = 1 V by electrical impedance sensing method (ECIS) on ECIS 8W1E electrodes. After confluence (colored lines), DMEM-based solutions containing glucose 17.33 g/L (Glc), dextran 14.9 g/L (D), dextran 14.9 g/L and sodium sulfate 25.99 g/L (D+S) and glucose 17.33 g/L and sulfate 25.99 g/L (Glc+S) were added at both pH. Data represent means of n = 3/condition.