Myocardial gene expression of inflammatory markers in wild type and CCR7 deficient mice during post-MI HF.

<p>Left sided panels show gene expression of monocyte chemoattractant protein (MCP)-1 (<b>A</b>) and tumor necrosis factor (TNF)α (<b>B</b>) in the LV one and six weeks following sham operation or MI in Wt (1w: n = 7+8; 6w: n = 5+7) and CCR7 deficient (1w: n = 6+9; 6w: n = 8+9) mice (numbers denote sham operation and post-MI HF, respectively). mRNA levels were quantified by real-time RT-PCR and are presented relative to the gene expression of GAPDH. Data are mean±SEM. *p<0.05, and ***p<0.001 versus sham operation in the same genotype. Right sided panels show representative images of immunohistochemical staining of CD45 as a pan-leukocyte marker (magnification 40×) in LV tissue from a Wt mouse (<b>C</b>) and a CCR7^−/− mouse (<b>D</b>) one week post-MI. Arrows indicate CD45-positive cells.