AMJ and AMJ-derived fractions induced a pro-apoptotic response in Jurkat cells.

<p>Cells were exposed either to increasing concentrations of AMJ, or AMJ-derived fractions (tested at 21.4 µg/mL, equivalent to the polyphenol content of AMJ 0.3% v/v), or various isolated pure products (100 µM) for 24 h. (A), (B) and (C) cell apoptosis rates were assessed by flow cytometry using the annexin V-FITC/PI apoptosis assay. (D) The expression level of key regulators of cell cycle and apoptosis was assessed by Western blot analysis. The data are representative of at least three independent experiments. Values are shown as means ± S.E.M. (n = 3); *, <i>P<0.05</i> versus respective control.