Lineage development and H3K27me3-accumulation on the X-chromosome in human embryos.

<p>Expression of GATA4, OCT4, and H3K27me3 in human day 5 pre-implantation and embryos that have been co-cultured with decidualized endometrial cells for 72 h (day 8). Foci of H3K27me3 are diagnostic for an inactivated X-chromosome. Arrowheads denote H3K27me3 foci. The third row panels are enlargements of the indicated area in the merge panel in the above row. Scale bars are 50 µm unless denoted otherwise.