Primers for bisulfite specific and methylation specific PCR.

<p>In <b>bold</b> are shown bases selectively matching to bisulfite-modified DNA:</p><p>C→<b>T</b> on sense target strand.</p><p>G→<b>A</b> on complementary strand.</p><p>Primers for bisulfite specific and methylation specific PCR are designed on bisulfite-converted, positive-sense strand of B19V genome and can be common and independent of CpG methylation status of target (0218, 0423), or be specific for unmethylated or methylated targets (0350 UnMet, 0350 Met).