Morphometric analysis and photomicrographs of the area of <i>T. cruzi</i> immunoreactions in the heart at 28 days after mice infection with metacyclic (MT; light gray bar) or blood trypomastigotes (BT; black bar) of <i>Trypanosoma cruzi</i>.

<p>Analysis of the area of <i>T. cruzi</i> amastigotes were identified by immunohistochemistry as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0032912#s4" target="_blank">Material and Methods</a>. The results are expressed as parasited area ± standard error. Photomicrographs of the heart parasitism demonstrating isolated amastigotes in MT and typical amastigote nests in BT.