Biodistribution of MNC in brain, spleen and lungs after IV administration of MNCs(10 million) at 24 hrs after stroke.

<p>MNCs were labeled with Q-tracker(green). These animals had undergone stroke, 24 hrs later were injected with MNCs IV and then sacrificed 1 hr afterwards. Sections were prepared at 1 hr after injection and counterstained with DAPI (blue). L-NAME was injected via IP 1 hr prior to MNC administration. N = 3 per group. (A): Representative microscopic pictures illustrating MNCs (green+blue) in animals pre-treated with L-NAME (top row) or saline (bottom row). Magnification: 400×. (B): A histogram illustrating the mean number of labeled MNCs per high power field in the brain, spleen, and lungs. Animals were pre-treated with L-NAME before IV injection of MNCs or animals were pre-treated with saline followed by IV injection of MNCs (‡p<0.05).