3D microscopy quantification of DR5 expression by CD4⁺ T cells.

<p><b>A.</b> CD4⁺ T cells were cultured overnight in presence or absence of PHA (2.5 µg/ml) and stained for DR5 (green), nuclear staining DAPI (blue) and CD4 (red) to study DR5 protein expression and localization. 10 cells per experiment were quantified for their DR5 spot number in 3 independent experiments for a total of 30 cells per condition (untreated or PHA-stimulated). White arrows show some examples of the DR5 spots that were counted <b>B.</b> Total number of DR5 spots in 30 untreated and 30 PHA-stimulated cells. <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0032874#s2" target="_blank">Results</a> show the number of DR5 spots per cell <b>C.</b> Percentage of membrane DR5 expression in 30 untreated and 30 PHA-stimulated cells. P values (<i>p</i>) were determined using a two-tailed Student's <i>t</i> test (p<0.05 one star, p<0.01 two stars, p<0.001 three stars).