<p>Cells were incubated overnight in media containing 5% charcoal-stripped FBS and were then transfected with an ERE-luciferase plasmid. After 4 hrs, drugs were added to the cells as indicated and luciferase levels were measured 18 hrs. later.</p
Background: Bioluminescent cancer models are widely used but detailed quantification of the lucifera...
<p>(A) Retroviral construct that contains the <i>effLuc</i> gene and Thy1.1 (CD90.1), linked with an...
After transfection, HUVEC cells were pretreated with AKT inhibitor IV (1 μM) and/or MEK1/2 inhibitor...
<p>For each time point, measured in days from the received time of individual clones covering a 60 m...
<p>(A) Luciferase assay established using HEK-293 cells co-transfected with a plasmid containing an ...
<p>MRC-5 cells were transfected with the pTA-Control plasmid by nucleofection and subsequently infec...
<p>G-418 resistant cell clones were seeded in 24-well tissue culture plates at 50–70% density in DME...
<p>(A) Luciferase reporter constructs with different inserts were transiently transfected in HEK293 ...
<p>A: Protocol for treating cells and measuring Luc activity. HeLa cells were transiently transfecte...
To date, multiplexing cell-based assay is essential for high-throughput screening of molecular targe...
<p>HEK-293 cells either tranfected with pcDNA-luc (CMV) or with the influenza replicon and pMono-luc...
<p>3T3-L1-pTet-On clone was transfected with pTRE-Tight-Luc plasmid. After 48 h post transfection, c...
<p>A: Determination of transfection efficiency of EL4 cells. EL4 cells were co-transfected with GFP ...
<p>ARE reporter cells were exposed to (15 µM) for 24 h. The value for cells treated with vehicle DMS...
Abstract: Firefly luciferase has proven to be a highly sensitive and quantitative reporter gene for ...
Background: Bioluminescent cancer models are widely used but detailed quantification of the lucifera...
<p>(A) Retroviral construct that contains the <i>effLuc</i> gene and Thy1.1 (CD90.1), linked with an...
After transfection, HUVEC cells were pretreated with AKT inhibitor IV (1 μM) and/or MEK1/2 inhibitor...
<p>For each time point, measured in days from the received time of individual clones covering a 60 m...
<p>(A) Luciferase assay established using HEK-293 cells co-transfected with a plasmid containing an ...
<p>MRC-5 cells were transfected with the pTA-Control plasmid by nucleofection and subsequently infec...
<p>G-418 resistant cell clones were seeded in 24-well tissue culture plates at 50–70% density in DME...
<p>(A) Luciferase reporter constructs with different inserts were transiently transfected in HEK293 ...
<p>A: Protocol for treating cells and measuring Luc activity. HeLa cells were transiently transfecte...
To date, multiplexing cell-based assay is essential for high-throughput screening of molecular targe...
<p>HEK-293 cells either tranfected with pcDNA-luc (CMV) or with the influenza replicon and pMono-luc...
<p>3T3-L1-pTet-On clone was transfected with pTRE-Tight-Luc plasmid. After 48 h post transfection, c...
<p>A: Determination of transfection efficiency of EL4 cells. EL4 cells were co-transfected with GFP ...
<p>ARE reporter cells were exposed to (15 µM) for 24 h. The value for cells treated with vehicle DMS...
Abstract: Firefly luciferase has proven to be a highly sensitive and quantitative reporter gene for ...
Background: Bioluminescent cancer models are widely used but detailed quantification of the lucifera...
<p>(A) Retroviral construct that contains the <i>effLuc</i> gene and Thy1.1 (CD90.1), linked with an...
After transfection, HUVEC cells were pretreated with AKT inhibitor IV (1 μM) and/or MEK1/2 inhibitor...