LC-MALDI MS-based identification of other interesting proteins proteins from MVs derived from acrosome reacted porcine sperm.

<p>Other interesting proteins identified in the MVs derived from bicarbonate capacitated and acrosome reacted cells based on similar criteria to <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0032603#pone-0032603-t001" target="_blank">Table 1</a>. These are proteins that have been previously reported to have roles in secondary sperm-zona binding functioning. Proteins that were not detected in the capacitated membrane cavitates (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0032603#pone-0032603-t003" target="_blank">Table 3</a>) and are exclusively present in the MVs based on these analyses are highlighted in grey. Most of these proteins have been previously reported to be acrosomal membrane specific <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0032603#pone.0032603-VanGestel2" target="_blank">[29]</a>. Protein ID was considered to be conclusive when two or more peptides were identified (e<0.05) (all proteins except the last protein in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0032603#pone-0032603-t002" target="_blank">Table 2</a>).</p><p>Protein ID was considered conclusive when two or more peptides were identified (e<0.05, all proteins above the line).</p>*<p>: indicate acrosomal specific membrane proteins.</p><p>Highlighted in grey: Proteins that are not detected in the capacitated membrane cavitates and are exclusively present in the mixed vesicles.</p><p>Highlighted in bold: SNARE interacting protein.