CNS-<i>Actb</i>KO mice exhibited hyperactivity and decreased performance in the Morris water maze.

<p>(<b>A</b>) CNS-<i>Actb</i>KO mice performed comparably to controls in a four day Rotarod assay experiment. Control and CNS-<i>Actb</i>KO animals were age matched and between 4–6 months old. Data plotted as mean ± standard error of the mean. (<b>B</b>) Five minute traces from control and CNS-<i>Actb</i>KO mice in an Open Field Activity Assay. (<b>C–D</b>) CNS-<i>Actb</i>KO mice traveled significantly greater distances and at significantly greater velocities than control mice. (<b>E</b>) Representative 30 second traces from control and CNS-<i>Actb</i>KO mice during the probe trial of a Morris water maze test. (<b>F</b>) CNS-<i>Actb</i>KO mice performed similarly to control mice with a cued platform and followed a similar learning curve until the final day of the hidden platform learning phase, at which time controls found the platform significantly faster. (<b>G–H</b>) In the probe trial, CNS-<i>Actb</i>KO mice spent a significantly smaller percentage of time in the platform quadrant and traveled significantly less distance in that quadrant. (<b>I</b>) CNS-<i>Actb</i>KO mice also crossed an area 50% larger than that of the actual platform significantly less times than control mice. <i>n</i> = 4 male mice for each genotype. * indicates <i>p</i><0.05, ** <i>p</i><0.01, *** <i>p</i><0.001.