Immunoblot analysis of four monoclonal antibodies and pre-existing polyclonal antibodies.

<p>Four kinds of monoclonal antibodies (N1-8, N1-216, N3-2, and N3-42) showed strong immunoreactivity for ∼200 kDa full-length human RB1CC1 and little cross-reactivity with other proteins in Western blot analysis. Two kinds of pre-existing polyclonal antibodies (N1-poly and N3-poly) were used to the blotting control. Four µg of protein lysates of HEK293 and the cells overexpressing RB1CC1 were applied to the analysis. An arrowhead shows the position of ∼200 kDa full-length human RB1CC1.