Effect of SPRC on pancreatitis-associated lung injury.

<p><b>A.</b> myeloperoxidase (MPO) activity. <b>B.</b> histology. Acute pancreatitis was induced by intraperitoneal (i.p.) administration of caerulein (50 µg/kg, hourly for 10 h). Mice were pre-treated with vehicle or SPRC (10 mg/kg, i.p.), either 3 h or 12 h before the induction of pancreatitis. One hour after the last caerulein injection, mice were killed by a lethal dose of pentobarbitone (50 mg/kg, i.p.) and lung MPO activity and histology were evaluated as described in “<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0032574#s2" target="_blank">Materials and methods</a>”. Key: CTRL, animals injected only with saline (i.p., hourly for 10 h) without the induction of pancreatitis; Veh+Cae, pre-treatment with vehicle (distilled water) before the induction of pancreatitis; SPRC 3 h+Cae, SPRC injected 3 h before the induction of pancreatitis; SPRC 12 h+Cae, SPRC injected 12 h before the induction of pancreatitis. <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0032574#s3" target="_blank">Results</a> shown are the mean ± s.e.m., n = 10 mice per group. ^*<i>P</i><0.05 when compared to the CTRL group; ^#<i>P</i><0.05 when compared to the Veh+Cae group. CTRL, control; Cae, caerulein; Veh, vehicle; SPRC, S-propargyl-cysteine.