<p>(A) Capillary electrophoresis of purified protein fractions. White stars indicate samples successfully crystallized and black stars represent solved structures. (B) Crystals of 5-keto-4-deoxyuronate isomerase crystallized from fractions of varying purity. Crystal quality was not always correlated with sample purity. (C) Resolution of the data collected versus percent purity of the starting sample based on quantification of protein concentrations by capillary gel electrophoresis with the Caliper system. Sample purity did not correlate with higher resolution data.</p
When starting a protein-crystallization project, scientists are faced with several unknowns. Amongst...
One essential prerequisite of any experiment involving a purified protein, such as interaction studi...
A fractionation technique should offer easy and good recovery of the sample with excellent resolutio...
Protein crystallisation screening involves the parallel testing of large numbers of candidate condit...
Structural biology and structural genomics projects routinely rely on recombinantly expressed protei...
Crystallization is the primary rate-limiting step in protein structure determination. It has been ou...
<p><i>A</i>, Electropherogram of 10–150 kDa molecular weight standards. The <i>numbers</i> indicate ...
AbstractThe importance of sample homogeneity and purity in protein crystallization is essential to o...
Strategies for growing protein crystals have for many years been essentially empirical, the protein,...
Protein characterization plays a role in two key aspects of structural proteomics. The first is the ...
Protein crystallization was discovered by chance nearly 200 years ago and was developed in the late ...
For every 100 purified proteins that enter crystallization trials, an average of 30 form crystals, a...
<p>Electropherograms of (a) <i>E. coli</i> supernatant after fractionation on G-25 column and (b) <i...
Protein characterization plays a role in two key aspects of structural proteomics. The first is the ...
The supply of many valuable proteins that have potential clinical or industrial use is often limited...
When starting a protein-crystallization project, scientists are faced with several unknowns. Amongst...
One essential prerequisite of any experiment involving a purified protein, such as interaction studi...
A fractionation technique should offer easy and good recovery of the sample with excellent resolutio...
Protein crystallisation screening involves the parallel testing of large numbers of candidate condit...
Structural biology and structural genomics projects routinely rely on recombinantly expressed protei...
Crystallization is the primary rate-limiting step in protein structure determination. It has been ou...
<p><i>A</i>, Electropherogram of 10–150 kDa molecular weight standards. The <i>numbers</i> indicate ...
AbstractThe importance of sample homogeneity and purity in protein crystallization is essential to o...
Strategies for growing protein crystals have for many years been essentially empirical, the protein,...
Protein characterization plays a role in two key aspects of structural proteomics. The first is the ...
Protein crystallization was discovered by chance nearly 200 years ago and was developed in the late ...
For every 100 purified proteins that enter crystallization trials, an average of 30 form crystals, a...
<p>Electropherograms of (a) <i>E. coli</i> supernatant after fractionation on G-25 column and (b) <i...
Protein characterization plays a role in two key aspects of structural proteomics. The first is the ...
The supply of many valuable proteins that have potential clinical or industrial use is often limited...
When starting a protein-crystallization project, scientists are faced with several unknowns. Amongst...
One essential prerequisite of any experiment involving a purified protein, such as interaction studi...
A fractionation technique should offer easy and good recovery of the sample with excellent resolutio...