MAVS co-immunoprecipitates with NS1 in epithelial cells infected with rA2- RSV or rA2.

<p>(<b>A</b>) HEp-2 cells were eithermock-infectedor infected with rA2 or rA2ΔNS1or rA2ΔNS2 at an MOI of 1. At 24 h p.i., total cell extracts were immunoprecipitated with anti-MAVS followed by western blot analysis using anti-NS1 or anti-MAVS antibodies. (<b>B</b>) Whole cell lysates from mock or rA2 infected HEp-2 cells were immunoblotted for RSV F protein using anti RSV F antibody (Chemicon MAB8262) (<i>right panel</i>). The lysates from mock or rA2 or rA2ΔNS1 were co-immunoprecipitated with anti-MAVS antibody and probed for MAVS and RSV F protein (<i>left panel</i>).