Immunogold electron microscopy labeling of Pax2 in the ONH.

<p>(all gold particles have been circled). (<b>A</b>) Pax2⁺ astrocyte with euchromatic nucleus (N) at 7 d post-injury. Inset: gold particle from the square. (<b>B</b>) Pax2⁺ astrocyte with euchromatic nucleus (N) at 21 d post-injury. Inset: gold particles from the square. (<b>C</b>) Composition of micrographs showing numerous glial cells (N: nucleus) among astrocyte processes with intermediate filaments (f), areas in degeneration (empty arrows) and dividing cells (ch: chromosomes) at 21 d post-injury. Close to the blood vessel (bv) there are granulocytes (G). Squares 1 and 2 are enlarged in D and E respectively. (<b>D</b>) Detail of a Pax2⁺ cell with a euchromatic nucleus (N). Inset: gold particles from the square. (<b>E</b>) Detail of intermediate filament packages (f). (<b>F</b>) Pax2⁺ astroblast with euchromatic nucleus (N). Upper left corner inset: gold particles from the bold square. Dotted square enlarged in upper right corner showing a doughnut-shaped mitochondria. Scale bars: A, C: 2500 nm; B, D–F: 1000 nm. Arrowheads: desmosomes; c: centrioles; CA: central artery; E: endothelium; ER: rough endoplasmic reticulum; f: intermediate filaments; In: indentations; mit: mitochondria; NE: nuclear envelope; Nuc: nucleolus; V: vesicles.