Gene expression and molecular characterization of goldfish <i>pax2a</i> mRNAs.

<p>(<b>A</b>) Expression of two <i>pax2a</i> mRNA variants (<i>pax2a_tv1</i> and <i>pax2a_tv2</i>) in goldfish optic nerve and 5 dpf zebrafish embryos by RT-PCR. MWM: molecular weight marker. (<b>B–C</b>) Comparison of two goldfish partial-length cDNAs, <i>pax2a_tv1</i> and <i>pax2a_tv2</i>, with their corresponding homologous in zebrafish. Alignment produced with the ClustalW program of the nucleotide sequences of <i>pax2a</i> of both cyprinidae species. The primers used for the molecular characterization are underlined and indicated in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0032348#pone-0032348-t002" target="_blank">Table 2</a>. The differences in the base pairs are shown with an arrow and the similarities with an asterisk. (<b>D–E</b>) Comparison of goldfish Pax2a_tv1/tv2 and their corresponding homologous in zebrafish. Alignment produced with the ClustalW program of the deduced amino acid sequences of Pax2a_tv1/tv2 of both cyprinidae species. The difference in the amino acids is shown with an arrow and the similarities with an asterisk. The octapeptide domain is marked in grey.