Double immunolabeling S100 (red)-Zn8 (green) and S100 (red)-GFAP (green) with DAPI (blue).

<p>(<b>A</b>) S100⁺ cells (arrows) and Zn8⁺ growing axons (empty arrows) in an <i>in toto</i> control retina, bv: blood vessels. PGZ: where this area is in the picture (broken line). (<b>B</b>) S100⁺ cell (arrow) in a control retina close to the PGZ. (<b>C</b>) S100⁺ cell (arrow) in control retina close to the ONH. (<b>D</b>) Detail of an S100⁺/GFAP⁻ cell in the control retina. (<b>E</b>) Detail of an S100⁺ cell with GFAP⁺ processes (arrow) in the control retina. (<b>F</b>) S100⁺ cells (arrows) in the injured PGZ at 7 d post-cryolesion. (<b>G</b>) High quantity of S100⁺ cells (arrows) in the retina close to the ONH at 7 d post injury. (<b>H</b>) S100⁺ cells (arrows) and Zn8⁺ regenerating axons (empty arrows) in an <i>in toto</i> retina 21 d after the cryolesion. The asterisk indicates an S100⁺ cell directing its processes to the inner retina. Confocal, maximum projection of 18 optical planes acquired with a step size of 1 micron. A 40× oil inmersion objective with a N.A. of 1.25 was used and the pinhole was set to 1.20 Airy units. (<b>I</b>) S100⁺ cells (arrows) in the injured PGZ at 15 d post-cryolesion. PGZ: where this area is in the picture. (<b>J</b>) S100⁺ cells (arrows) in the retina close to the ONH 15 d post injury. (<b>K</b>) Statistical analysis of the S100⁺ cell density in the retina during regeneration. The cap and two caps indicate the significant (∧0.01