Macroscopic and histological comparison of livers from C57Bl6, alb-SREBP-1aΔP and alb-SREBP-1a mice.

<p>Male mice of each genotype (C57Bl6, alb-SREBP1aΔP, alb-SREBP-1a (n = 20 per genotype)) were housed as groups of 4 under standard conditions with unlimited access to water and regular chow (13.0 MJ/kg: 53% carbohydrates, 11% fat, 36% protein). (<b>A</b>) Livers of a C57Bl6 mouse (left), alb-SREBP-1aΔP (middle) or alb-SREBP-1a (right). All photographs were taken with the same magnification. (<b>B</b>) Liver tissue of the Lobus caudatus, Lobus sinister- and Lobus dexter lateralis were fixed in 4% paraformaldehyd/PBS and embedded in paraffin with automated standard histological procedures. (I) Standard hematoxylin and eosin staining was performed on 3 µm deparaffinized sections. (II) PAS staining was performed to determine glycogen contend. (III) The tissues were also used for cryofixation and Oil-red-O staining was used for lipid visualization. (IV) Fibers and extra cellular matrix were visualized using the “van Gierson kit” to determine tissue integrity. The overview magnification is 1∶10 and details are shown in 1∶80 magnification.