Radiation increases autofluorescence in a dose- and time- dependent fashion.

<p>DC2.4 cells were irradiated or not under standard culture conditions and analyzed by FACS. <b>A</b>) Forward-side scatter dot blot and Fl-1 histogram of untreated cells and cells at 24 h after treatment with 10 Gy. <b>B</b>) (left) Overlay histograms of FL-1 fluorescence in 10 Gy-irradiated cells analyzed at 24, 48 and 72 h and (right) overlay histogram of FL-1 fluorescence after 0, 2, 5 and 10 Gy and analyzed at 48 h. <b>C</b>) Mean FL-1 fluorescence intensity normalized to control of n = 2±s.d. in DC2.4 treated with radiation ranging from 0.005 to 0.5 Gy and analyzed at 48 h after exposure. *p<0.05.