Effect of pre- or post-HD serum on the expression of collagen-IV and elastin.

<p>HUVEC were incubated with culture medium supplemented with 20% pre- or post- HD serum. 6, 12, or 24 h later total RNA was extracted from the cells, RT-PCR reactions were performed using specific primers for Collagen IV, Elastin or GAPDH mRNAs, the PCR products were analyzed in agarose gels and quantified. Data are expressed as mean ± SEM of three independent experiments. ^*, ^** and ^*** represent p<0.05, p<0.01 and p<0.001 respectively.