Transposon mutants with reduced hemolytic activity on sheep blood agar.

a<p>Based on Rapid Annotation using Subsystem Technology (RAST) of de novo assembled genome fragments from PSU3316.</p>b<p><i>E</i> values determined using NCBI BLASTN using the full open reading frame of PSU3316.</p>c<p>Control challenges with wild-type PSU3316 or transposant mutants with wild-type hemolysis activity exhibited mortality of 100% at the challenge dose.</p>d<p>Hemolysis attenuated mutants exhibited variable mortality that was statistically different from wild-type as evaluated by Fisher's exact test (<i>p</i><0.05).