Multiphoton Z-Stack images of microglia cells immunohistochemically stained by Iba-1 to show the cell bodies and cell processes.

<p>The panels show a Z-Stack of images while the panels to the right and top demonstrate the level of the image in the stack. (A): partially activated microglia with a few activated cells. The cross hairs show the location of the cell and the upper and right panels show the depth of the cell. (B): activated microglial cells as by increased size and more circular shape size. (C): fully activated microglia with several microglia cells in stage 5R. Note: the cells cover the full thickness of the histological slide and although some cells are located close to each other, they can clearly delineated from each other by the Iba-1 label in the cell walls. Scale bar: 10 µm.