ICV ghrelin administration activates the HPA axis.

<p>Panel <b>A</b> shows comparative values of RT-qPCR for CRF mRNA in the PVN micro-dissected punches obtained from vehicle-treated and ghrelin-treated mice sacrificed at different time points. Data is shown as mRNA levels of CRF relative to the housekeeping gene Cyclophilin A, calculated by the comparative threshold cycle (Ct) method. Panel <b>B</b> shows comparative values of specific ELISA for plasma corticosterone obtained from vehicle- and ghrelin-treated mice sacrificed at different time points. Data represent the mean±SEM. *p≤0.05. **p≤0.01. ***p≤0.001.