MALDI-IMS analysis of anthocyanins in black rice sections.

<p>A, Black rice section stained with toluidine blue O after MALDI-IMS analysis of the ions at <i>m/z</i> 449. B, Localization pattern of the ions at <i>m/z</i> 449. C, Localization pattern of the ions at <i>m/z</i> 822 corresponding to PC (diacyl C36:3), which marks the nucellus epidermis/aleurone layer. D, Localization pattern of the ions at <i>m/z</i> 496 corresponding to LPC (C16:0), which marks the endosperm. E, Merged ion image of <i>m/z</i> 449 (red), <i>m/z</i> 822 (green) and <i>m/z</i> 496 (white). F, Black rice section stained with toluidine blue O after MALDI-IMS analysis of the ions at <i>m/z</i> 463. G, Localization pattern of the ions at <i>m/z</i> 463. H, Localization pattern of the ions at <i>m/z</i> 822 corresponding to PC (diacyl C36:3). I, Localization pattern of the ions at <i>m/z</i> 496 corresponding to LPC (C16:0). J, Merged ion image of <i>m/z</i> 463 (red), <i>m/z</i> 822 (green) and <i>m/z</i> 496 (white). Scale bar: 1.0 mm.