The effects of CsA and Tac on cytokine production from memory CD4⁺ T cells.

<p>Memory CD4⁺ T cells were stimulated with PMA and ionomycin in the absence or presence of CsA or Tac. Fluorescence profiles showed distinct cytokine productions from mature cells, and the production of all cytokines investigated here was strikingly suppressed with the addition of CsA or Tac, even at the lower concentration. Representative figures of ten independent experiments are shown (A). The percentages of IFN-γ⁺CD4⁺ T cells (B), IL-4⁺CD4⁺ T cells (C) or IL-17⁺CD4⁺ T cells (D) within the CD4⁺ T cell population were significantly suppressed with the addition of CsA or Tac. Data are expressed as the mean ± SEM.