The effects of CsA and Tac on the differentiation of naïve CD4⁺ T cells into cytokine-producing mature cells (Th1/Th2/Th17).

<p>Flow cytometric analysis showed abundant cytokine production from CD45RA⁻CD45RO⁺CD4⁺ T cells. Representative figures of ten independent experiments are shown (A). Addition of CsA or Tac lowered the percentage of CD45RA⁻CD45RO⁺IFN-γ⁺ cells significantly compared with the control group (B). The percentages of CD45RA⁻CD45RO⁺IL-4⁺ cells and CD45RA⁻CD45RO⁺IL-17⁺ cells within the CD4⁺ T cell population were also decreased compared with the control group (C,D). Data are expressed as the mean ± SEM.