IL-17 has opposing effects on the infiltration of CD11b+ macrophages and CD8+ T cells in DMBA/TPA treated skin.

<p>Mice were treated with DMBA and TPA as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0032126#pone-0032126-g001" target="_blank">figure 1</a> and skin tissues were harvested at the end of the experiment (26 weeks). A). Frozen tissue sections were stained with CD11b antibodies (brown) and counterstained with hematoxylin (blue, upper panels) or with anti-CD8 antibodies (green) and counterstained with a fluorescence dye DAPI (blue, lower panel). Photos were taken microscopically (20× objective). Numbers of positive cells were counted microscopically. Islets show the enlarged areas of circled squares with positive cells. Graphs show the mean number of positive cells per field +/− SEM, <i>n</i> = 10, * p<0.05, ** P<0.01. B). Gr-1 and CD11b double positive cells in spleens were stained with fluorescence labeled antibodies and positive cells were analyzed in a flow cytometer. The numbers in the right upper quadrant indicate the percent of positive cells in whole spleen cells. C). Statistical analysis of GR-1/CD11b+ (myeloid derived suppressor cells), CD19/B220+ (B cells), CD8+ and CD4+ T cells in spleens (mean +/− SEM, n = 8, ** P<0.01). Data are representative of two independent experiments.