FTL_0883 deletion mutant, ΔFTL_0883, elicits maturation of DCs and is attenuated for growth similar to 13B47.

<p>Human (A–E) and murine DCs (F–G) were cultured with either LVS, 13B47, ΔFTL_0883, or ΔFTL_0883::pJH1- FTL_0883 at an MOI of 10 (A–B, D, and F) or 500 followed by gentamicin treatment (C–E, and G). For cytokines, supernatants were harvested at 24 hours (A, C, and F) or the indicated time points (B), and IL-12p40 and TNF-α were measured by ELISA. For flow cytometry experiments (D), DCs were harvested 24 hours post infection and GMFIs for CD80 and CD86 were measured. For gentamicin protection assays (E, G), DCs were infected with LVS strains at an MOI of 500 and then lysed at indicated time points to enumerate intracellular bacteria. Data are presented as the mean ± SEM from at least two independent experiments. Statistically significant differences between groups were determined by one (A, C, D, and F) or two-way ANOVA (B, E, and G), followed by Bonferroni comparison of means (*, p<0.05; **, p<0.01; ***, p<0.001). BLD = below limits of detection of the ELISA.