TPA induced promotion of tumor growth is abrogated in IL-17R-/- mice.

<p>A). TPA induced inflammation enhances tumor growth. Wild type C57Bl/6 mice were treated epicutaneously with TPA or acetone (vehicle controls) every other day for a total of 5 applications. The mice were then inoculated subcutaneously with EG7 tumor cells. Tumor growth was monitored (n = 5). B). TPA induced inflammation increases tumor specific IL-17 producing cells. At the end of experiments, the draining lymph node lymphocytes were collected from EG7 tumor bearing mice that were treated with TPA or acetone. The cells stimulated with OVA pulsed BM-DC for 4 days. Concentrations of cytokines in supernatants were measured by ELISA (n = 4). Naïve T cells with OVA pulsed BM-DC (Naïve) and immune T cells with BM-DC without OVA (Neg) served as controls in the cultures. C). TPA induced tumor promotion is abrogated in IL-17R-/- mice. Wild type or IL-17R-/- were epicutaneously treated with TPA or acetone every other day for a total of 5 applications. The mice were then inoculated with EG7 tumor cells and tumor growth was monitored (n = 5). Data are representative of 2–3 independent experiments (mean +/− SEM, * P<0.05).