Digestion of 1 µg mutated phagemid DNA library pools with HindIII and SacII.

Covalent Modification of Bacteriophage T4 DNA Inhibits CRISPR-

Frederic D. Bushmana

August 2016

ABSTRACT The genomic DNAs of tailed bacteriophages are commonly modified by the attachment of chemic...

Southern blot analysis of PVL phage genomic DNA from strain 68111 and strain ATCC49775.

Zhi Li (72309)
Dennis L. Stevens (340325)
Stephanie M. Hamilton (340326)
Tanyalak Parimon (340327)
Yongsheng Ma (340328)
Angela M. Kearns (340329)
Richard W. Ellis (340330)
Amy E. Bryant (340332)

February 2013

Phage DNA from MSSA68111 (Lanes designated “6”) and from strain ATCC49775 (Lanes “A”) was digeste...

Practical Method for Isolation of Phage Deletion Mutants

Diana Gutiérrez

January 2018

The growing concern about multi-drug resistant pathogenic bacteria has led to a renewed interest in ...

Phage DNA following digestion with DNase I and RNase A (5 µL loaded) (A), Lane M: XXL DNA Ladder GeneOn; Lane 1: uncut VP-2 phage nucleic acid; Lane 2: DNase I digested VP-2 phage nucleic acid; Lane 3: RNase A digested VP-2 phage nucleic acid; Lane 4: uncut VA-1 phage nucleic acid; Lane 5: DNase I digested VA-1 phage nucleic acid; Lane 6: RNase A digested VA-1 phage nucleic acid.

Yolanda J. Silva (668425)
Liliana Costa (668426)
Carla Pereira (668427)
Cristiana Mateus (668428)
Ângela Cunha (2794681)
Ricardo Calado (169371)
Newton C. M. Gomes (188736)
Miguel A. Pardo (668429)
Igor Hernandez (668430)
Adelaide Almeida (236827)

December 2014

Agarose gel (0.8%) showing electrophoretic patterns of S1 Nuclease restriction digestion of phage...

PFGE of S1 nuclease-digested total DNA.

Ståle Tofteland (387723)
Umaer Naseer (147233)
Jan Helge Lislevand (387724)
Arnfinn Sundsfjord (147251)
Ørjan Samuelsen (115985)

March 2013

Lanes 1 and 20, phage λ DNA ladder (concatemers of 48.5 kb); lane 2, K47-25; lane 3, K48-58; lane...

Restriction digestion patterns of phage ArV2 genomic DNA.

Eugenijus Šimoliūnas (401210)
Laura Kaliniene (401211)
Miroslav Stasilo (647917)
Lidija Truncaitė (401212)
Aurelija Zajančkauskaitė (401213)
Juozas Staniulis (401214)
Juozas Nainys (647918)
Algirdas Kaupinis (401215)
Mindaugas Valius (401217)
Rolandas Meškys (4799871)

October 2014

Lanes: 1 – ArV2 gDNA (nonrestricted); 2 – EcoRII; 3 – NotI; 4 – MboI; 5 – BamHI; 6 – Marker GeneR...

Library production and counter-selection scheme.

Michael H. Norris (360610)
Yun Heacock-Kang (4691773)
Jan Zarzycki-Siek (360608)
Andrew P. Bluhm (416240)
Ian A. McMillan (416241)
Herbert P. Schweizer (131693)
Tung T. Hoang (360611)

December 2017

(A) Plasmid map of an in-lab created pBBR-FOSGAT and strategy for creating the fosmid library. <i...

Protection of DNA from Dnase I digestion.

Yan-Feng Liu (326557)
Nan Zhang (46264)
Xi Liu (142824)
Xinquan Wang (211905)
Zhi-Xin Wang (156984)
Yuanyuan Chen (165812)
Hong-Wei Yao (326558)
Meng Ge (156979)
Xian-Ming Pan (40954)

February 2013

(a) Sso10b and its triple mutant. and (b) Mth10b and its triple mutant: Lane 1, pla...

Data Corresponding to Figure 1e

Pawan Kumar (6386009)

March 2019

pUC19 plasmid was denatured using 0.5 N NaOH, pH was neutralized using 0.5 M HCl, and DNA was incuba...

Table_1_Type II Restriction of Bacteriophage DNA With 5hmdU-Derived Base Modifications.docx

Kiersten Flodman (6526703)
Rebecca Tsai (6526706)
Michael Y. Xu (6526709)
Ivan R. Corrêa (3922445)
Alyssa Copelas (6526712)
Yan-Jiun Lee (1316118)
Ming-Qun Xu (174409)
Peter Weigele (27032)
Shuang-yong Xu (14559)

March 2019

To counteract bacterial defense systems, bacteriophages (phages) make extensive base modifications (...

Construction of the mutant libraries.

Nicholas C. Wu (549942)
C. Anders Olson (549941)
Yushen Du (549945)
Shuai Le (412109)
Kevin Tran (519050)
Roland Remenyi (270101)
Danyang Gong (763969)
Laith Q. Al-Mawsawi (307260)
Hangfei Qi (549940)
Ting-Ting Wu (1693231)
Ren Sun (82856)

July 2015

(A) A schematic representation of the fitness profiling experiment is shown. A 240 bp insert was ...

Estimation of DNA MTase activity of the F17S and G19D M.SssI mutants by restriction enzyme protection assay.

Ildikó Stier (474839)
Antal Kiss (474840)

October 2013

<div>Lambda phage DNA was incubated with different concentrations of WT and mutant M.SssI in the ...

Protection of DNA by M.CbeI or M.HaeIII from digestion in vitro.

Daehwan Chung (310593)
Joel Farkas (310595)
Jennifer R. Huddleston (310597)
Estefania Olivar (310599)
Janet Westpheling (38371)

February 2013

In each panel, lane 1 is unmethylated plasmid DNA (pUC18) isolated from E. coli (dam<su...

The recombinant plasmid pET28a(+)–SjPGM (A) and pET28a(+)–SjRAD23 (B) were identified by enzyme digestion.

Min Zhang (111999)
Zhiqiang Fu (124249)
Changjian Li (694868)
Yanhui Han (422668)
Xiaodan Cao (694869)
Hongxiao Han (216412)
Yantao Liu (694870)
Ke Lu (124270)
Yang Hong (124259)
Jiaojiao Lin (124273)

February 2015

Lane 1: The blank plasmid pET28a(+). Lane 2: The recombinant plasmid pET28a(+)–SjPGM or pET28a(+)...

Investigating Nuclease Processing with Variably Phosphorothioated Cassettes. A)

Joshua A. Mosberg (138589)
Christopher J. Gregg (138592)
Marc J. Lajoie (138595)
Harris H. Wang (138598)
George M. Church (102039)

September 2012

Diagram of the variably phosphorothioated (VPT) cassette series. Homology arms to lacZ are...

Covalent Modification of Bacteriophage T4 DNA Inhibits CRISPR-

Frederic D. Bushmana

August 2016

ABSTRACT The genomic DNAs of tailed bacteriophages are commonly modified by the attachment of chemic...

Southern blot analysis of PVL phage genomic DNA from strain 68111 and strain ATCC49775.

Zhi Li (72309)
Dennis L. Stevens (340325)
Stephanie M. Hamilton (340326)
Tanyalak Parimon (340327)
Yongsheng Ma (340328)
Angela M. Kearns (340329)
Richard W. Ellis (340330)
Amy E. Bryant (340332)

February 2013

Phage DNA from MSSA68111 (Lanes designated “6”) and from strain ATCC49775 (Lanes “A”) was digeste...

Practical Method for Isolation of Phage Deletion Mutants

Diana Gutiérrez

January 2018

The growing concern about multi-drug resistant pathogenic bacteria has led to a renewed interest in ...

Phage DNA following digestion with DNase I and RNase A (5 µL loaded) (A), Lane M: XXL DNA Ladder GeneOn; Lane 1: uncut VP-2 phage nucleic acid; Lane 2: DNase I digested VP-2 phage nucleic acid; Lane 3: RNase A digested VP-2 phage nucleic acid; Lane 4: uncut VA-1 phage nucleic acid; Lane 5: DNase I digested VA-1 phage nucleic acid; Lane 6: RNase A digested VA-1 phage nucleic acid.

Yolanda J. Silva (668425)
Liliana Costa (668426)
Carla Pereira (668427)
Cristiana Mateus (668428)
Ângela Cunha (2794681)
Ricardo Calado (169371)
Newton C. M. Gomes (188736)
Miguel A. Pardo (668429)
Igor Hernandez (668430)
Adelaide Almeida (236827)

December 2014

Agarose gel (0.8%) showing electrophoretic patterns of S1 Nuclease restriction digestion of phage...

PFGE of S1 nuclease-digested total DNA.

Ståle Tofteland (387723)
Umaer Naseer (147233)
Jan Helge Lislevand (387724)
Arnfinn Sundsfjord (147251)
Ørjan Samuelsen (115985)

March 2013

Lanes 1 and 20, phage λ DNA ladder (concatemers of 48.5 kb); lane 2, K47-25; lane 3, K48-58; lane...

Restriction digestion patterns of phage ArV2 genomic DNA.

Eugenijus Šimoliūnas (401210)
Laura Kaliniene (401211)
Miroslav Stasilo (647917)
Lidija Truncaitė (401212)
Aurelija Zajančkauskaitė (401213)
Juozas Staniulis (401214)
Juozas Nainys (647918)
Algirdas Kaupinis (401215)
Mindaugas Valius (401217)
Rolandas Meškys (4799871)

October 2014

Lanes: 1 – ArV2 gDNA (nonrestricted); 2 – EcoRII; 3 – NotI; 4 – MboI; 5 – BamHI; 6 – Marker GeneR...

Library production and counter-selection scheme.

Michael H. Norris (360610)
Yun Heacock-Kang (4691773)
Jan Zarzycki-Siek (360608)
Andrew P. Bluhm (416240)
Ian A. McMillan (416241)
Herbert P. Schweizer (131693)
Tung T. Hoang (360611)

December 2017

(A) Plasmid map of an in-lab created pBBR-FOSGAT and strategy for creating the fosmid library. <i...

Protection of DNA from Dnase I digestion.

Yan-Feng Liu (326557)
Nan Zhang (46264)
Xi Liu (142824)
Xinquan Wang (211905)
Zhi-Xin Wang (156984)
Yuanyuan Chen (165812)
Hong-Wei Yao (326558)
Meng Ge (156979)
Xian-Ming Pan (40954)

February 2013

(a) Sso10b and its triple mutant. and (b) Mth10b and its triple mutant: Lane 1, pla...

Data Corresponding to Figure 1e

Pawan Kumar (6386009)

March 2019

pUC19 plasmid was denatured using 0.5 N NaOH, pH was neutralized using 0.5 M HCl, and DNA was incuba...

Table_1_Type II Restriction of Bacteriophage DNA With 5hmdU-Derived Base Modifications.docx

Kiersten Flodman (6526703)
Rebecca Tsai (6526706)
Michael Y. Xu (6526709)
Ivan R. Corrêa (3922445)
Alyssa Copelas (6526712)
Yan-Jiun Lee (1316118)
Ming-Qun Xu (174409)
Peter Weigele (27032)
Shuang-yong Xu (14559)

March 2019

To counteract bacterial defense systems, bacteriophages (phages) make extensive base modifications (...

Construction of the mutant libraries.

Nicholas C. Wu (549942)
C. Anders Olson (549941)
Yushen Du (549945)
Shuai Le (412109)
Kevin Tran (519050)
Roland Remenyi (270101)
Danyang Gong (763969)
Laith Q. Al-Mawsawi (307260)
Hangfei Qi (549940)
Ting-Ting Wu (1693231)
Ren Sun (82856)

July 2015

(A) A schematic representation of the fitness profiling experiment is shown. A 240 bp insert was ...

Estimation of DNA MTase activity of the F17S and G19D M.SssI mutants by restriction enzyme protection assay.

Ildikó Stier (474839)
Antal Kiss (474840)

October 2013

<div>Lambda phage DNA was incubated with different concentrations of WT and mutant M.SssI in the ...

Protection of DNA by M.CbeI or M.HaeIII from digestion in vitro.

Daehwan Chung (310593)
Joel Farkas (310595)
Jennifer R. Huddleston (310597)
Estefania Olivar (310599)
Janet Westpheling (38371)

February 2013

In each panel, lane 1 is unmethylated plasmid DNA (pUC18) isolated from E. coli (dam<su...

The recombinant plasmid pET28a(+)–SjPGM (A) and pET28a(+)–SjRAD23 (B) were identified by enzyme digestion.

Min Zhang (111999)
Zhiqiang Fu (124249)
Changjian Li (694868)
Yanhui Han (422668)
Xiaodan Cao (694869)
Hongxiao Han (216412)
Yantao Liu (694870)
Ke Lu (124270)
Yang Hong (124259)
Jiaojiao Lin (124273)

February 2015

Lane 1: The blank plasmid pET28a(+). Lane 2: The recombinant plasmid pET28a(+)–SjPGM or pET28a(+)...

Investigating Nuclease Processing with Variably Phosphorothioated Cassettes. A)

Joshua A. Mosberg (138589)
Christopher J. Gregg (138592)
Marc J. Lajoie (138595)
Harris H. Wang (138598)
George M. Church (102039)

September 2012

Diagram of the variably phosphorothioated (VPT) cassette series. Homology arms to lacZ are...

Covalent Modification of Bacteriophage T4 DNA Inhibits CRISPR-

Frederic D. Bushmana

August 2016

ABSTRACT The genomic DNAs of tailed bacteriophages are commonly modified by the attachment of chemic...

Southern blot analysis of PVL phage genomic DNA from strain 68111 and strain ATCC49775.

Zhi Li (72309)
Dennis L. Stevens (340325)
Stephanie M. Hamilton (340326)
Tanyalak Parimon (340327)
Yongsheng Ma (340328)
Angela M. Kearns (340329)
Richard W. Ellis (340330)
Amy E. Bryant (340332)

February 2013

Phage DNA from MSSA68111 (Lanes designated “6”) and from strain ATCC49775 (Lanes “A”) was digeste...

Practical Method for Isolation of Phage Deletion Mutants

Diana Gutiérrez

January 2018

The growing concern about multi-drug resistant pathogenic bacteria has led to a renewed interest in ...

Digestion of 1 µg mutated phagemid DNA library pools with HindIII and SacII.

Abstract

Extracted data

Digestion of 1 µg mutated phagemid DNA library pools with HindIII and SacII.

Abstract

Extracted data

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