Rescue of PKCθ expression in hematopoietic cells in <i>mdx/θ^−/−</i> mice, by bone marrow transplantation.

<p>(<b>A</b>) PCR analysis of BM cell suspension genomic DNA derived from <i>mdx/θ^{−/−BMmdx/θ−/−}</i> and from <i>mdx/θ^−/−BMmdx</i>, as indicated. The reappearance of the 400 kb PCR product from the PKCθ gene in <i>mdx/θ^−/−BMmdx</i> is shown. (<b>B</b>) Western Blot analysis of PKCθ, PKCδ and PKCα expression in total protein extract from thymi derived from <i>mdx/θ^{−/−BM mdx/θ−/−}</i> and from <i>mdx/θ^−/−BMmdx</i>. (<b>C</b>) Immunofluorescence analysis of PKCθ-expressing cells (green) in the spleens derived from <i>mdx/θ^{−/−BMmdx/θ−/−}</i> and from <i>mdx/θ^−/−BMmdx</i>. Hoechst was used to counterstain the nuclei (blue).